| 1 | Tue 13 Sep 2016 | 0-2 v ChesterChester 2-0 Guiseley | National League |
| 2 | Sat 17 Sep 2016 | 1-1 v Maidstone UnitedMaidstone United 1-1 Guiseley | National League |
| 3 | Sat 24 Sep 2016 | 1-2 v Macclesfield TownGuiseley 1-2 Macclesfield Town | National League |
| 4 | Sat 1 Oct 2016 | 0-1 v Sutton UnitedSutton United 1-0 Guiseley | National League |
| 5 | Tue 4 Oct 2016 | 196-1 v York CityGuiseley 6-1 York City | National League |
| 6 | Sat 8 Oct 2016 | 2-1 v SouthportGuiseley 2-1 Southport | National League |
| 7 | Sat 22 Oct 2016 | 0-3 v Forest Green RoversForest Green Rovers 3-0 Guiseley | National League |
| 8 | Tue 25 Oct 2016 | 1-1 v GatesheadGuiseley 1-1 Gateshead | National League |
| 9 | Tue 22 Nov 2016 | 0-3 v BarrowBarrow 3-0 Guiseley | National League |
| 10 | Sat 26 Nov 2016 | 3-1 v Boreham WoodGuiseley 3-1 Boreham Wood | National League |
| 11 | Tue 29 Nov 2016 | 1-1 v York CityYork City 1-1 Guiseley | National League |
| 12 | Sat 3 Dec 2016 | 1-1 v ChesterGuiseley 1-1 Chester | National League |
| 13 | Sat 17 Dec 2016 | 0-0 v WokingWoking 0-0 Guiseley | National League |
| 14 | Mon 26 Dec 2016 | 2-1 v Lincoln CityGuiseley 2-1 Lincoln City | National League |
| 15 | Sun 1 Jan 2017 | 1-3 v Lincoln CityLincoln City 3-1 Guiseley | National League |
| 16 | Sat 7 Jan 2017 | 2-1 v Maidstone UnitedGuiseley 2-1 Maidstone United | National League |
| 17 | Sat 21 Jan 2017 | 2-1 v Macclesfield TownMacclesfield Town 1-2 Guiseley | National League |
| 18 | Sat 11 Feb 2017 | 1-1 v EastleighGuiseley 1-1 Eastleigh | National League |
| 19 | Tue 14 Feb 2017 | 2-1 v Sutton UnitedGuiseley 2-1 Sutton United | National League |
| 20 | Sat 18 Feb 2017 | 2-1 v Dagenham & RedbridgeDagenham & Redbridge 1-2 Guiseley | National League |
| 21 | Sat 25 Feb 2017 | 1-2 v North Ferriby UnitedGuiseley 1-2 North Ferriby United | National League |
| 22 | Tue 28 Feb 2017 | 1-0 v BarrowGuiseley 1-0 Barrow | National League |
| 23 | Sat 4 Mar 2017 | 2-1 v Torquay UnitedTorquay United 1-2 Guiseley | National League |
| 24 | Sat 11 Mar 2017 | 1-0 v Aldershot TownGuiseley 1-0 Aldershot Town | National League |
| 25 | Sat 18 Mar 2017 | 0-0 v Boreham WoodBoreham Wood 0-0 Guiseley | National League |
| 26 | Tue 21 Mar 2017 | 1-1 v GatesheadGateshead 1-1 Guiseley | National League |
| 27 | Sat 25 Mar 2017 | 0-4 v Dover AthleticGuiseley 0-4 Dover Athletic | National League |
| 28 | Sat 1 Apr 2017 | 1-0 v SouthportSouthport 0-1 Guiseley | National League |
| 29 | Sat 8 Apr 2017 | 240-1 v Forest Green RoversGuiseley 0-1 Forest Green Rovers | National League |
| 30 | Fri 14 Apr 2017 | 240-2 v Braintree TownBraintree Town 2-0 Guiseley | National League |
| 31 | Mon 17 Apr 2017 | 241-2 v Tranmere RoversGuiseley 1-2 Tranmere Rovers | National League |
| 32 | Sat 22 Apr 2017 | 241-1 v BromleyBromley 1-1 Guiseley | National League |
| 33 | Sat 29 Apr 2017 | 241-1 v Solihull MoorsGuiseley 1-1 Solihull Moors | National League |
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