| 1 | Sat 6 Aug 2016 | 61-2 v EastleighEastleigh 2-1 Guiseley | National League |
| 2 | Tue 9 Aug 2016 | 62-3 v WrexhamGuiseley 2-3 Wrexham | National League |
| 3 | Sat 13 Aug 2016 | 60-2 v Dagenham & RedbridgeGuiseley 0-2 Dagenham & Redbridge | National League |
| 4 | Tue 16 Aug 2016 | 62-3 v North Ferriby UnitedNorth Ferriby United 3-2 Guiseley | National League |
| 5 | Sat 20 Aug 2016 | 62-3 v Solihull MoorsSolihull Moors 3-2 Guiseley | National League |
| 6 | Mon 29 Aug 2016 | 60-1 v Tranmere RoversTranmere Rovers 1-0 Guiseley | National League |
| 7 | Sat 3 Sep 2016 | 60-0 v Braintree TownGuiseley 0-0 Braintree Town | National League |
| 8 | Sat 10 Sep 2016 | 61-1 v WokingGuiseley 1-1 Woking | National League |
| 9 | Tue 13 Sep 2016 | 60-2 v ChesterChester 2-0 Guiseley | National League |
| 10 | Sat 17 Sep 2016 | 61-1 v Maidstone UnitedMaidstone United 1-1 Guiseley | National League |
| 11 | Sat 24 Sep 2016 | 61-2 v Macclesfield TownGuiseley 1-2 Macclesfield Town | National League |
| 12 | Sat 1 Oct 2016 | 60-1 v Sutton UnitedSutton United 1-0 Guiseley | National League |
| 13 | Tue 4 Oct 2016 | 66-1 v York CityGuiseley 6-1 York City | National League |
| 14 | Sat 8 Oct 2016 | 62-1 v SouthportGuiseley 2-1 Southport | National League |
| 15 | Sat 22 Oct 2016 | 60-3 v Forest Green RoversForest Green Rovers 3-0 Guiseley | National League |
| 16 | Tue 25 Oct 2016 | 61-1 v GatesheadGuiseley 1-1 Gateshead | National League |
| 17 | Sat 29 Oct 2016 | 60-1 v Aldershot TownAldershot Town 1-0 Guiseley | National League |
| 18 | Sat 21 Jan 2017 | 62-1 v Macclesfield TownMacclesfield Town 1-2 Guiseley | National League |
| 19 | Sat 11 Feb 2017 | 61-1 v EastleighGuiseley 1-1 Eastleigh | National League |
| 20 | Tue 14 Feb 2017 | 62-1 v Sutton UnitedGuiseley 2-1 Sutton United | National League |
| 21 | Sat 18 Feb 2017 | 62-1 v Dagenham & RedbridgeDagenham & Redbridge 1-2 Guiseley | National League |
| 22 | Sat 25 Feb 2017 | 61-2 v North Ferriby UnitedGuiseley 1-2 North Ferriby United | National League |
| 23 | Tue 28 Feb 2017 | 61-0 v BarrowGuiseley 1-0 Barrow | National League |
| 24 | Sat 4 Mar 2017 | 62-1 v Torquay UnitedTorquay United 1-2 Guiseley | National League |
| 25 | Tue 21 Mar 2017 | 61-1 v GatesheadGateshead 1-1 Guiseley | National League |
| 26 | Sat 25 Mar 2017 | 60-4 v Dover AthleticGuiseley 0-4 Dover Athletic | National League |
| 27 | Sat 1 Apr 2017 | 61-0 v SouthportSouthport 0-1 Guiseley | National League |
| 28 | Sat 8 Apr 2017 | 60-1 v Forest Green RoversGuiseley 0-1 Forest Green Rovers | National League |
| 29 | Fri 14 Apr 2017 | 60-2 v Braintree TownBraintree Town 2-0 Guiseley | National League |
| 30 | Mon 17 Apr 2017 | 61-2 v Tranmere RoversGuiseley 1-2 Tranmere Rovers | National League |
| 31 | Sat 22 Apr 2017 | 61-1 v BromleyBromley 1-1 Guiseley | National League |
| 32 | Sat 29 Apr 2017 | 61-1 v Solihull MoorsGuiseley 1-1 Solihull Moors | National League |
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