| 1 | Sat 17 Sep 2016 | 31-1 v Maidstone UnitedMaidstone United 1-1 Guiseley | National League |
| 2 | Sat 24 Sep 2016 | 31-2 v Macclesfield TownGuiseley 1-2 Macclesfield Town | National League |
| 3 | Sat 1 Oct 2016 | 30-1 v Sutton UnitedSutton United 1-0 Guiseley | National League |
| 4 | Tue 4 Oct 2016 | 36-1 v York CityGuiseley 6-1 York City | National League |
| 5 | Sat 8 Oct 2016 | 32-1 v SouthportGuiseley 2-1 Southport | National League |
| 6 | Sat 22 Oct 2016 | 30-3 v Forest Green RoversForest Green Rovers 3-0 Guiseley | National League |
| 7 | Tue 25 Oct 2016 | 31-1 v GatesheadGuiseley 1-1 Gateshead | National League |
| 8 | Sat 29 Oct 2016 | 30-1 v Aldershot TownAldershot Town 1-0 Guiseley | National League |
| 9 | Sat 12 Nov 2016 | 32-0 v Torquay UnitedGuiseley 2-0 Torquay United | National League |
| 10 | Sat 19 Nov 2016 | 30-2 v Dover AthleticDover Athletic 2-0 Guiseley | National League |
| 11 | Tue 22 Nov 2016 | 30-3 v BarrowBarrow 3-0 Guiseley | National League |
| 12 | Sat 26 Nov 2016 | 33-1 v Boreham WoodGuiseley 3-1 Boreham Wood | National League |
| 13 | Tue 29 Nov 2016 | 31-1 v York CityYork City 1-1 Guiseley | National League |
| 14 | Sat 3 Dec 2016 | 31-1 v ChesterGuiseley 1-1 Chester | National League |
| 15 | Sat 17 Dec 2016 | 30-0 v WokingWoking 0-0 Guiseley | National League |
| 16 | Mon 26 Dec 2016 | 32-1 v Lincoln CityGuiseley 2-1 Lincoln City | National League |
| 17 | Sun 1 Jan 2017 | 31-3 v Lincoln CityLincoln City 3-1 Guiseley | National League |
| 18 | Sat 21 Jan 2017 | 32-1 v Macclesfield TownMacclesfield Town 1-2 Guiseley | National League |
| 19 | Sat 11 Feb 2017 | 31-1 v EastleighGuiseley 1-1 Eastleigh | National League |
| 20 | Tue 14 Feb 2017 | 32-1 v Sutton UnitedGuiseley 2-1 Sutton United | National League |
| 21 | Sat 18 Feb 2017 | 32-1 v Dagenham & RedbridgeDagenham & Redbridge 1-2 Guiseley | National League |
| 22 | Sat 25 Feb 2017 | 31-2 v North Ferriby UnitedGuiseley 1-2 North Ferriby United | National League |
| 23 | Tue 21 Mar 2017 | 31-1 v GatesheadGateshead 1-1 Guiseley | National League |
| 24 | Sat 25 Mar 2017 | 30-4 v Dover AthleticGuiseley 0-4 Dover Athletic | National League |
| 25 | Sat 1 Apr 2017 | 31-0 v SouthportSouthport 0-1 Guiseley | National League |
| 26 | Sat 8 Apr 2017 | 200-1 v Forest Green RoversGuiseley 0-1 Forest Green Rovers | National League |
| 27 | Sat 29 Apr 2017 | 31-1 v Solihull MoorsGuiseley 1-1 Solihull Moors | National League |
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