| 1 | Sat 20 Aug 2016 | 2-3 v Solihull MoorsSolihull Moors 3-2 Guiseley | National League |
| 2 | Sat 27 Aug 2016 | 1-4 v BromleyGuiseley 1-4 Bromley | National League |
| 3 | Mon 29 Aug 2016 | 0-1 v Tranmere RoversTranmere Rovers 1-0 Guiseley | National League |
| 4 | Sat 3 Sep 2016 | 0-0 v Braintree TownGuiseley 0-0 Braintree Town | National League |
| 5 | Sat 10 Sep 2016 | 1-1 v WokingGuiseley 1-1 Woking | National League |
| 6 | Tue 13 Sep 2016 | 0-2 v ChesterChester 2-0 Guiseley | National League |
| 7 | Sat 17 Sep 2016 | 1-1 v Maidstone UnitedMaidstone United 1-1 Guiseley | National League |
| 8 | Sat 24 Sep 2016 | 1-2 v Macclesfield TownGuiseley 1-2 Macclesfield Town | National League |
| 9 | Sat 1 Oct 2016 | 0-1 v Sutton UnitedSutton United 1-0 Guiseley | National League |
| 10 | Tue 4 Oct 2016 | 226-1 v York CityGuiseley 6-1 York City | National League |
| 11 | Sat 8 Oct 2016 | 2-1 v SouthportGuiseley 2-1 Southport | National League |
| 12 | Sat 22 Oct 2016 | 0-3 v Forest Green RoversForest Green Rovers 3-0 Guiseley | National League |
| 13 | Tue 25 Oct 2016 | 1-1 v GatesheadGuiseley 1-1 Gateshead | National League |
| 14 | Sat 29 Oct 2016 | 0-1 v Aldershot TownAldershot Town 1-0 Guiseley | National League |
| 15 | Sat 12 Nov 2016 | 2-0 v Torquay UnitedGuiseley 2-0 Torquay United | National League |
| 16 | Sat 19 Nov 2016 | 0-2 v Dover AthleticDover Athletic 2-0 Guiseley | National League |
| 17 | Tue 22 Nov 2016 | 0-3 v BarrowBarrow 3-0 Guiseley | National League |
| 18 | Sat 26 Nov 2016 | 3-1 v Boreham WoodGuiseley 3-1 Boreham Wood | National League |
| 19 | Tue 29 Nov 2016 | 1-1 v York CityYork City 1-1 Guiseley | National League |
| 20 | Sat 3 Dec 2016 | 1-1 v ChesterGuiseley 1-1 Chester | National League |
| 21 | Mon 26 Dec 2016 | 2-1 v Lincoln CityGuiseley 2-1 Lincoln City | National League |
| 22 | Sat 25 Mar 2017 | 0-4 v Dover AthleticGuiseley 0-4 Dover Athletic | National League |
| 23 | Sat 1 Apr 2017 | 1-0 v SouthportSouthport 0-1 Guiseley | National League |
| 24 | Sat 8 Apr 2017 | 150-1 v Forest Green RoversGuiseley 0-1 Forest Green Rovers | National League |
| 25 | Fri 14 Apr 2017 | 0-2 v Braintree TownBraintree Town 2-0 Guiseley | National League |
| 26 | Mon 17 Apr 2017 | 1-2 v Tranmere RoversGuiseley 1-2 Tranmere Rovers | National League |
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