| 1 | Sat 18 Aug 2012 | 4-1 v HistonHiston 1-4 Guiseley | Blue Square Bet North |
| 2 | Tue 21 Aug 2012 | 7-1 v DroylsdenGuiseley 7-1 Droylsden | Blue Square Bet North |
| 3 | Sat 25 Aug 2012 | 2-0 v Gainsborough TrinityGuiseley 2-0 Gainsborough Trinity | Blue Square Bet North |
| 4 | Mon 27 Aug 2012 | 2-0 v WorkingtonWorkington 0-2 Guiseley | Blue Square Bet North |
| 5 | Sat 1 Sep 2012 | 3-1 v Gloucester CityGuiseley 3-1 Gloucester City | Blue Square Bet North |
| 6 | Tue 4 Sep 2012 | 2-0 v Colwyn BayColwyn Bay 0-2 Guiseley | Blue Square Bet North |
| 7 | Sat 8 Sep 2012 | 0-4 v ChesterChester 4-0 Guiseley | Blue Square Bet North |
| 8 | Tue 18 Sep 2012 | 2-0 v Harrogate TownGuiseley 2-0 Harrogate Town | Blue Square Bet North |
| 9 | Sat 29 Sep 2012 | 3-0 v Oxford CityOxford City 0-3 Guiseley | Blue Square Bet North |
| 10 | Tue 2 Oct 2012 | 1-0 v Stalybridge CelticGuiseley 1-0 Stalybridge Celtic | Blue Square Bet North |
| 11 | Sat 13 Oct 2012 | 0-2 v Solihull MoorsSolihull Moors 2-0 Guiseley | Blue Square Bet North |
| 12 | Sat 27 Oct 2012 | 2-1 v Vauxhall MotorsGuiseley 2-1 Vauxhall Motors | Blue Square Bet North |
| 13 | Tue 30 Oct 2012 | 3-1 v AltrinchamAltrincham 1-3 Guiseley | Blue Square Bet North |
| 14 | Sat 3 Nov 2012 | 32-2 v BarrowGuiseley 2-2 Barrow | FA Cup with Budweiser First Round |
| 15 | Tue 13 Nov 2012 | 30-1 v BarrowBarrow 1-0 Guiseley | FA Cup with Budweiser First Round Replay |
| 16 | Sat 17 Nov 2012 | 33-1 v Boston UnitedBoston United 1-3 Guiseley | Blue Square Bet North |
| 17 | Tue 20 Nov 2012 | 33-3 v Worcester CityGuiseley 3-3 Worcester City | Blue Square Bet North |
| 18 | Sat 1 Dec 2012 | 33-0 v Hinckley UnitedHinckley United 0-3 Guiseley | Blue Square Bet North |
| 19 | Wed 26 Dec 2012 | 31-0 v Bradford Park AvenueGuiseley 1-0 Bradford Park Avenue | Blue Square Bet North |
| 20 | Tue 1 Jan 2013 | 33-1 v Bradford Park AvenueBradford Park Avenue 1-3 Guiseley | Blue Square Bet North |
| 21 | Sat 5 Jan 2013 | 35-2 v Bishop's StortfordBishop's Stortford 2-5 Guiseley | Blue Square Bet North |
| 22 | Tue 8 Jan 2013 | 32-1 v Corby TownGuiseley 2-1 Corby Town | Blue Square Bet North |
| 23 | Sat 12 Jan 2013 | 31-0 v Oxford CityGuiseley 1-0 Oxford City | Blue Square Bet North |
| 24 | Sat 9 Feb 2013 | 32-1 v Boston UnitedGuiseley 2-1 Boston United | Blue Square Bet North |
| 25 | Tue 12 Feb 2013 | 32-2 v Gloucester CityGloucester City 2-2 Guiseley | Blue Square Bet North |
| 26 | Tue 19 Feb 2013 | 32-2 v Colwyn BayGuiseley 2-2 Colwyn Bay | Blue Square Bet North |
| 27 | Sat 23 Feb 2013 | 31-0 v Worcester CityWorcester City 0-1 Guiseley | Blue Square Bet North |
| 28 | Sat 2 Mar 2013 | 30-2 v Brackley TownGuiseley 0-2 Brackley Town | Blue Square Bet North |
| 29 | Wed 6 Mar 2013 | 33-2 v Corby TownCorby Town 2-3 Guiseley | Blue Square Bet North |
| 30 | Sat 9 Mar 2013 | 33-0 v DroylsdenDroylsden 0-3 Guiseley | Blue Square Bet North |
| 31 | Sat 16 Mar 2013 | 31-0 v Solihull MoorsGuiseley 1-0 Solihull Moors | Blue Square Bet North |
| 32 | Sat 30 Mar 2013 | 32-1 v Gainsborough TrinityGainsborough Trinity 1-2 Guiseley | Blue Square Bet North |
| 33 | Mon 1 Apr 2013 | 32-0 v WorkingtonGuiseley 2-0 Workington | Blue Square Bet North |
| 34 | Wed 3 Apr 2013 | 31-1 v FC Halifax TownGuiseley 1-1 FC Halifax Town | Blue Square Bet North |
| 35 | Sat 6 Apr 2013 | 30-1 v Brackley TownBrackley Town 1-0 Guiseley | Blue Square Bet North |
| 36 | Thu 11 Apr 2013 | 31-1 v AltrinchamGuiseley 1-1 Altrincham | Blue Square Bet North |
| 37 | Sat 13 Apr 2013 | 32-1 v ChesterGuiseley 2-1 Chester | Blue Square Bet North |
| 38 | Sat 20 Apr 2013 | 31-1 v FC Halifax TownFC Halifax Town 1-1 Guiseley | Blue Square Bet North |
| 39 | Tue 23 Apr 2013 | 32-1 v Harrogate TownHarrogate Town 1-2 Guiseley | Blue Square Bet North |
| 40 | Sat 4 May 2013 | 30-2 v FC Halifax TownGuiseley 0-2 FC Halifax Town | Blue Square Bet North Play-off Semi Final |
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