| 1 | Sat 17 Aug 2013 | 1-3 v North Ferriby UnitedNorth Ferriby United 3-1 Gloucester City | Skrill North |
| 2 | Tue 20 Aug 2013 | 2-1 v Worcester CityGloucester City 2-1 Worcester City | Skrill North |
| 3 | Sat 24 Aug 2013 | 1-2 v AFC Telford UnitedGloucester City 1-2 AFC Telford United | Skrill North |
| 4 | Mon 26 Aug 2013 | 1-0 v LeamingtonLeamington 0-1 Gloucester City | Skrill North |
| 5 | Sat 31 Aug 2013 | 1-1 v GuiseleyGloucester City 1-1 Guiseley | Skrill North |
| 6 | Sat 7 Sep 2013 | 0-0 v BarrowBarrow 0-0 Gloucester City | Skrill North |
| 7 | Sat 14 Sep 2013 | 2-4 v WorkingtonWorkington 4-2 Gloucester City | Skrill North |
| 8 | Tue 17 Sep 2013 | 2-2 v Brackley TownGloucester City 2-2 Brackley Town | Skrill North |
| 9 | Sat 21 Sep 2013 | 1-3 v Boston UnitedGloucester City 1-3 Boston United | Skrill North |
| 10 | Tue 24 Sep 2013 | 0-2 v AltrinchamAltrincham 2-0 Gloucester City | Skrill North |
| 11 | Sat 5 Oct 2013 | 1-4 v Hednesford TownHednesford Town 4-1 Gloucester City | Skrill North |
| 12 | Sat 19 Oct 2013 | 2-2 v Stockport CountyStockport County 2-2 Gloucester City | Skrill North |
| 13 | Tue 29 Oct 2013 | 0-3 v Solihull MoorsGloucester City 0-3 Solihull Moors | Skrill North |
| 14 | Sat 2 Nov 2013 | 1-3 v Bradford Park AvenueBradford Park Avenue 3-1 Gloucester City | Skrill North |
| 15 | Sat 9 Nov 2013 | 30-2 v Fleetwood TownGloucester City 0-2 Fleetwood Town | FA Cup with Budweiser First Round |
| 16 | Sat 23 Nov 2013 | 32-3 v Vauxhall MotorsVauxhall Motors 3-2 Gloucester City | Skrill North |
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