| 1 | Sat 9 Nov 2013 | 10-2 v Fleetwood TownGloucester City 0-2 Fleetwood Town | FA Cup with Budweiser First Round |
| 2 | Tue 12 Nov 2013 | 12-3 v Colwyn BayGloucester City 2-3 Colwyn Bay | Skrill North |
| 3 | Sat 23 Nov 2013 | 12-3 v Vauxhall MotorsVauxhall Motors 3-2 Gloucester City | Skrill North |
| 4 | Sat 7 Dec 2013 | 5-1 v Hednesford TownGloucester City 5-1 Hednesford Town | Skrill North |
| 5 | Tue 10 Dec 2013 | 2-0 v AltrinchamGloucester City 2-0 Altrincham | Skrill North |
| 6 | Sat 14 Dec 2013 | 2-4 v Harrogate TownHarrogate Town 4-2 Gloucester City | Skrill North |
| 7 | Sat 21 Dec 2013 | 0-1 v Gainsborough TrinityGloucester City 0-1 Gainsborough Trinity | Skrill North |
| 8 | Thu 26 Dec 2013 | 0-1 v Oxford CityOxford City 1-0 Gloucester City | Skrill North |
| 9 | Sat 28 Dec 2013 | 1-0 v Worcester CityWorcester City 0-1 Gloucester City | Skrill North |
| 10 | Sat 11 Jan 2014 | 2-3 v Bradford Park AvenueGloucester City 2-3 Bradford Park Avenue | Skrill North |
| 11 | Sat 18 Jan 2014 | 2-2 v Stalybridge CelticStalybridge Celtic 2-2 Gloucester City | Skrill North |
| 12 | Tue 18 Feb 2014 | 1-3 v BarrowGloucester City 1-3 Barrow | Skrill North |
| 13 | Tue 4 Mar 2014 | 2-0 v HistonGloucester City 2-0 Histon | Skrill North |
| 14 | Sat 8 Mar 2014 | 2-0 v Stockport CountyGloucester City 2-0 Stockport County | Skrill North |
| 15 | Sat 15 Mar 2014 | 3-3 v Gainsborough TrinityGainsborough Trinity 3-3 Gloucester City | Skrill North |
| 16 | Tue 18 Mar 2014 | 3-1 v Brackley TownBrackley Town 1-3 Gloucester City | Skrill North |
| 17 | Sat 22 Mar 2014 | 1-0 v Stalybridge CelticGloucester City 1-0 Stalybridge Celtic | Skrill North |
| 18 | Tue 25 Mar 2014 | 3-1 v HistonHiston 1-3 Gloucester City | Skrill North |
| 19 | Sat 29 Mar 2014 | 0-2 v Boston UnitedBoston United 2-0 Gloucester City | Skrill North |
| 20 | Tue 1 Apr 2014 | 0-2 v Oxford CityGloucester City 0-2 Oxford City | Skrill North |
| 21 | Sun 6 Apr 2014 | 5-2 v Harrogate TownGloucester City 5-2 Harrogate Town | Skrill North |
| 22 | Sat 12 Apr 2014 | 2-2 v Vauxhall MotorsGloucester City 2-2 Vauxhall Motors | Skrill North |
| 23 | Tue 15 Apr 2014 | 1-1 v WorkingtonGloucester City 1-1 Workington | Skrill North |
| 24 | Sat 19 Apr 2014 | 1-2 v AFC Telford UnitedAFC Telford United 2-1 Gloucester City | Skrill North |
| 25 | Mon 21 Apr 2014 | 3-3 v LeamingtonGloucester City 3-3 Leamington | Skrill North |
| 26 | Sat 26 Apr 2014 | 1-3 v GuiseleyGuiseley 3-1 Gloucester City | Skrill North |
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