| 1 | Sat 17 Aug 2013 | 1-3 v North Ferriby UnitedNorth Ferriby United 3-1 Gloucester City | Skrill North |
| 2 | Tue 20 Aug 2013 | 2-1 v Worcester CityGloucester City 2-1 Worcester City | Skrill North |
| 3 | Mon 26 Aug 2013 | 1-0 v LeamingtonLeamington 0-1 Gloucester City | Skrill North |
| 4 | Sat 31 Aug 2013 | 1-1 v GuiseleyGloucester City 1-1 Guiseley | Skrill North |
| 5 | Sat 21 Sep 2013 | 1-3 v Boston UnitedGloucester City 1-3 Boston United | Skrill North |
| 6 | Tue 24 Sep 2013 | 0-2 v AltrinchamAltrincham 2-0 Gloucester City | Skrill North |
| 7 | Sat 5 Oct 2013 | 1-4 v Hednesford TownHednesford Town 4-1 Gloucester City | Skrill North |
| 8 | Sat 19 Oct 2013 | 2-2 v Stockport CountyStockport County 2-2 Gloucester City | Skrill North |
| 9 | Tue 29 Oct 2013 | 0-3 v Solihull MoorsGloucester City 0-3 Solihull Moors | Skrill North |
| 10 | Sat 2 Nov 2013 | 1-3 v Bradford Park AvenueBradford Park Avenue 3-1 Gloucester City | Skrill North |
| 11 | Sat 9 Nov 2013 | 40-2 v Fleetwood TownGloucester City 0-2 Fleetwood Town | FA Cup with Budweiser First Round |
| 12 | Sat 23 Nov 2013 | 42-3 v Vauxhall MotorsVauxhall Motors 3-2 Gloucester City | Skrill North |
| 13 | Sat 7 Dec 2013 | 5-1 v Hednesford TownGloucester City 5-1 Hednesford Town | Skrill North |
| 14 | Tue 10 Dec 2013 | 2-0 v AltrinchamGloucester City 2-0 Altrincham | Skrill North |
| 15 | Sat 14 Dec 2013 | 2-4 v Harrogate TownHarrogate Town 4-2 Gloucester City | Skrill North |
| 16 | Sat 21 Dec 2013 | 0-1 v Gainsborough TrinityGloucester City 0-1 Gainsborough Trinity | Skrill North |
| 17 | Thu 26 Dec 2013 | 0-1 v Oxford CityOxford City 1-0 Gloucester City | Skrill North |
| 18 | Sat 28 Dec 2013 | 1-0 v Worcester CityWorcester City 0-1 Gloucester City | Skrill North |
| 19 | Sat 11 Jan 2014 | 2-3 v Bradford Park AvenueGloucester City 2-3 Bradford Park Avenue | Skrill North |
| 20 | Sat 18 Jan 2014 | 2-2 v Stalybridge CelticStalybridge Celtic 2-2 Gloucester City | Skrill North |
| 21 | Tue 18 Feb 2014 | 1-3 v BarrowGloucester City 1-3 Barrow | Skrill North |
| 22 | Sat 22 Feb 2014 | 1-1 v North Ferriby UnitedGloucester City 1-1 North Ferriby United | Skrill North |
| 23 | Tue 25 Feb 2014 | 1-1 v Colwyn BayColwyn Bay 1-1 Gloucester City | Skrill North |
| 24 | Sat 1 Mar 2014 | 1-2 v Solihull MoorsSolihull Moors 2-1 Gloucester City | Skrill North |
| 25 | Tue 4 Mar 2014 | 2-0 v HistonGloucester City 2-0 Histon | Skrill North |
| 26 | Sat 8 Mar 2014 | 2-0 v Stockport CountyGloucester City 2-0 Stockport County | Skrill North |
| 27 | Sat 15 Mar 2014 | 3-3 v Gainsborough TrinityGainsborough Trinity 3-3 Gloucester City | Skrill North |
| 28 | Tue 18 Mar 2014 | 3-1 v Brackley TownBrackley Town 1-3 Gloucester City | Skrill North |
| 29 | Sat 22 Mar 2014 | 1-0 v Stalybridge CelticGloucester City 1-0 Stalybridge Celtic | Skrill North |
| 30 | Tue 25 Mar 2014 | 3-1 v HistonHiston 1-3 Gloucester City | Skrill North |
| 31 | Sat 29 Mar 2014 | 0-2 v Boston UnitedBoston United 2-0 Gloucester City | Skrill North |
| 32 | Tue 1 Apr 2014 | 0-2 v Oxford CityGloucester City 0-2 Oxford City | Skrill North |
| 33 | Sun 6 Apr 2014 | 5-2 v Harrogate TownGloucester City 5-2 Harrogate Town | Skrill North |
| 34 | Sat 12 Apr 2014 | 2-2 v Vauxhall MotorsGloucester City 2-2 Vauxhall Motors | Skrill North |
| 35 | Tue 15 Apr 2014 | 1-1 v WorkingtonGloucester City 1-1 Workington | Skrill North |
| 36 | Sat 19 Apr 2014 | 1-2 v AFC Telford UnitedAFC Telford United 2-1 Gloucester City | Skrill North |
| 37 | Mon 21 Apr 2014 | 3-3 v LeamingtonGloucester City 3-3 Leamington | Skrill North |
| 38 | Sat 26 Apr 2014 | 1-3 v GuiseleyGuiseley 3-1 Gloucester City | Skrill North |
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