| 1 | Sat 7 Dec 2013 | 5-1 v Hednesford TownGloucester City 5-1 Hednesford Town | Skrill North |
| 2 | Tue 10 Dec 2013 | 2-0 v AltrinchamGloucester City 2-0 Altrincham | Skrill North |
| 3 | Sat 14 Dec 2013 | 2-4 v Harrogate TownHarrogate Town 4-2 Gloucester City | Skrill North |
| 4 | Sat 21 Dec 2013 | 0-1 v Gainsborough TrinityGloucester City 0-1 Gainsborough Trinity | Skrill North |
| 5 | Sat 28 Dec 2013 | 1-0 v Worcester CityWorcester City 0-1 Gloucester City | Skrill North |
| 6 | Sat 11 Jan 2014 | 2-3 v Bradford Park AvenueGloucester City 2-3 Bradford Park Avenue | Skrill North |
| 7 | Sat 18 Jan 2014 | 2-2 v Stalybridge CelticStalybridge Celtic 2-2 Gloucester City | Skrill North |
| 8 | Tue 18 Feb 2014 | 1-3 v BarrowGloucester City 1-3 Barrow | Skrill North |
| 9 | Tue 25 Feb 2014 | 1-1 v Colwyn BayColwyn Bay 1-1 Gloucester City | Skrill North |
| 10 | Sat 1 Mar 2014 | 1-2 v Solihull MoorsSolihull Moors 2-1 Gloucester City | Skrill North |
| 11 | Tue 4 Mar 2014 | 2-0 v HistonGloucester City 2-0 Histon | Skrill North |
| 12 | Tue 25 Mar 2014 | 3-1 v HistonHiston 1-3 Gloucester City | Skrill North |
| 13 | Sat 29 Mar 2014 | 0-2 v Boston UnitedBoston United 2-0 Gloucester City | Skrill North |
| 14 | Sat 12 Apr 2014 | 2-2 v Vauxhall MotorsGloucester City 2-2 Vauxhall Motors | Skrill North |
| 15 | Tue 15 Apr 2014 | 1-1 v WorkingtonGloucester City 1-1 Workington | Skrill North |
| 16 | Mon 21 Apr 2014 | 3-3 v LeamingtonGloucester City 3-3 Leamington | Skrill North |
| 17 | Sat 26 Apr 2014 | 1-3 v GuiseleyGuiseley 3-1 Gloucester City | Skrill North |
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