| 1 | Sat 17 Aug 2013 | 1-3 v North Ferriby UnitedNorth Ferriby United 3-1 Gloucester City | Skrill North |
| 2 | Sat 24 Aug 2013 | 1-2 v AFC Telford UnitedGloucester City 1-2 AFC Telford United | Skrill North |
| 3 | Mon 26 Aug 2013 | 1-0 v LeamingtonLeamington 0-1 Gloucester City | Skrill North |
| 4 | Sat 31 Aug 2013 | 1-1 v GuiseleyGloucester City 1-1 Guiseley | Skrill North |
| 5 | Sat 7 Sep 2013 | 0-0 v BarrowBarrow 0-0 Gloucester City | Skrill North |
| 6 | Sat 14 Sep 2013 | 2-4 v WorkingtonWorkington 4-2 Gloucester City | Skrill North |
| 7 | Tue 17 Sep 2013 | 2-2 v Brackley TownGloucester City 2-2 Brackley Town | Skrill North |
| 8 | Sat 21 Sep 2013 | 1-3 v Boston UnitedGloucester City 1-3 Boston United | Skrill North |
| 9 | Tue 24 Sep 2013 | 0-2 v AltrinchamAltrincham 2-0 Gloucester City | Skrill North |
| 10 | Sat 5 Oct 2013 | 1-4 v Hednesford TownHednesford Town 4-1 Gloucester City | Skrill North |
| 11 | Sat 19 Oct 2013 | 2-2 v Stockport CountyStockport County 2-2 Gloucester City | Skrill North |
| 12 | Tue 29 Oct 2013 | 0-3 v Solihull MoorsGloucester City 0-3 Solihull Moors | Skrill North |
| 13 | Sat 9 Nov 2013 | 80-2 v Fleetwood TownGloucester City 0-2 Fleetwood Town | FA Cup with Budweiser First Round |
| 14 | Tue 12 Nov 2013 | 82-3 v Colwyn BayGloucester City 2-3 Colwyn Bay | Skrill North |
| 15 | Sat 7 Dec 2013 | 5-1 v Hednesford TownGloucester City 5-1 Hednesford Town | Skrill North |
| 16 | Tue 10 Dec 2013 | 2-0 v AltrinchamGloucester City 2-0 Altrincham | Skrill North |
| 17 | Sat 14 Dec 2013 | 2-4 v Harrogate TownHarrogate Town 4-2 Gloucester City | Skrill North |
| 18 | Sat 21 Dec 2013 | 0-1 v Gainsborough TrinityGloucester City 0-1 Gainsborough Trinity | Skrill North |
| 19 | Sat 28 Dec 2013 | 1-0 v Worcester CityWorcester City 0-1 Gloucester City | Skrill North |
| 20 | Sat 11 Jan 2014 | 2-3 v Bradford Park AvenueGloucester City 2-3 Bradford Park Avenue | Skrill North |
| 21 | Sat 18 Jan 2014 | 2-2 v Stalybridge CelticStalybridge Celtic 2-2 Gloucester City | Skrill North |
| 22 | Tue 18 Feb 2014 | 1-3 v BarrowGloucester City 1-3 Barrow | Skrill North |
| 23 | Sat 22 Feb 2014 | 1-1 v North Ferriby UnitedGloucester City 1-1 North Ferriby United | Skrill North |
| 24 | Tue 25 Feb 2014 | 1-1 v Colwyn BayColwyn Bay 1-1 Gloucester City | Skrill North |
| 25 | Sat 1 Mar 2014 | 1-2 v Solihull MoorsSolihull Moors 2-1 Gloucester City | Skrill North |
| 26 | Tue 4 Mar 2014 | 2-0 v HistonGloucester City 2-0 Histon | Skrill North |
| 27 | Sat 8 Mar 2014 | 2-0 v Stockport CountyGloucester City 2-0 Stockport County | Skrill North |
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