| 1 | Sat 18 Aug 2012 | 1-0 v Gainsborough TrinityGainsborough Trinity 0-1 Gloucester City | Blue Square Bet North |
| 2 | Tue 21 Aug 2012 | 0-1 v Oxford CityGloucester City 0-1 Oxford City | Blue Square Bet North |
| 3 | Sat 25 Aug 2012 | 1-2 v HistonHiston 2-1 Gloucester City | Blue Square Bet North |
| 4 | Tue 4 Sep 2012 | 1-4 v Brackley TownGloucester City 1-4 Brackley Town | Blue Square Bet North |
| 5 | Sat 15 Sep 2012 | 0-5 v FC Halifax TownFC Halifax Town 5-0 Gloucester City | Blue Square Bet North |
| 6 | Sat 29 Sep 2012 | 0-1 v DroylsdenDroylsden 1-0 Gloucester City | Blue Square Bet North |
| 7 | Sat 8 Dec 2012 | 170-1 v WorkingtonGloucester City 0-1 Workington | Blue Square Bet North |
| 8 | Sat 15 Dec 2012 | 172-1 v Vauxhall MotorsVauxhall Motors 1-2 Gloucester City | Blue Square Bet North |
| 9 | Wed 26 Dec 2012 | 171-0 v Worcester CityWorcester City 0-1 Gloucester City | Blue Square Bet North |
| 10 | Tue 1 Jan 2013 | 174-2 v Worcester CityGloucester City 4-2 Worcester City | Blue Square Bet North |
| 11 | Sat 2 Feb 2013 | 170-2 v AltrinchamAltrincham 2-0 Gloucester City | Blue Square Bet North |
| 12 | Tue 5 Feb 2013 | 171-0 v Brackley TownBrackley Town 0-1 Gloucester City | Blue Square Bet North |
| 13 | Sat 9 Feb 2013 | 171-0 v Bradford Park AvenueGloucester City 1-0 Bradford Park Avenue | Blue Square Bet North |
| 14 | Tue 12 Feb 2013 | 172-2 v GuiseleyGloucester City 2-2 Guiseley | Blue Square Bet North |
| 15 | Tue 19 Feb 2013 | 171-2 v FC Halifax TownGloucester City 1-2 FC Halifax Town | Blue Square Bet North |
| 16 | Sat 23 Feb 2013 | 170-2 v Harrogate TownGloucester City 0-2 Harrogate Town | Blue Square Bet North |
| 17 | Sat 2 Mar 2013 | 171-0 v WorkingtonWorkington 0-1 Gloucester City | Blue Square Bet North |
| 18 | Tue 5 Mar 2013 | 170-4 v Boston UnitedBoston United 4-0 Gloucester City | Blue Square Bet North |
| 19 | Sat 9 Mar 2013 | 174-1 v Hinckley UnitedGloucester City 4-1 Hinckley United | Blue Square Bet North |
| 20 | Sat 30 Mar 2013 | 171-1 v HistonGloucester City 1-1 Histon | Blue Square Bet North |
| 21 | Mon 1 Apr 2013 | 173-2 v Solihull MoorsSolihull Moors 2-3 Gloucester City | Blue Square Bet North |
| 22 | Wed 3 Apr 2013 | 172-1 v Bishop's StortfordBishop's Stortford 1-2 Gloucester City | Blue Square Bet North |
| 23 | Sat 6 Apr 2013 | 171-1 v Vauxhall MotorsGloucester City 1-1 Vauxhall Motors | Blue Square Bet North |
| 24 | Tue 9 Apr 2013 | 170-1 v Harrogate TownHarrogate Town 1-0 Gloucester City | Blue Square Bet North |
| 25 | Sat 13 Apr 2013 | 170-4 v Stalybridge CelticStalybridge Celtic 4-0 Gloucester City | Blue Square Bet North |
| 26 | Tue 16 Apr 2013 | 171-2 v Gainsborough TrinityGloucester City 1-2 Gainsborough Trinity | Blue Square Bet North |
| 27 | Sat 20 Apr 2013 | 170-1 v Corby TownGloucester City 0-1 Corby Town | Blue Square Bet North |
| 28 | Sat 27 Apr 2013 | 170-1 v Colwyn BayColwyn Bay 1-0 Gloucester City | Blue Square Bet North |
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