| 1 | Tue 21 Aug 2012 | 0-1 v Oxford CityGloucester City 0-1 Oxford City | Blue Square Bet North |
| 2 | Sat 25 Aug 2012 | 1-2 v HistonHiston 2-1 Gloucester City | Blue Square Bet North |
| 3 | Tue 4 Sep 2012 | 1-4 v Brackley TownGloucester City 1-4 Brackley Town | Blue Square Bet North |
| 4 | Sat 15 Sep 2012 | 0-5 v FC Halifax TownFC Halifax Town 5-0 Gloucester City | Blue Square Bet North |
| 5 | Tue 2 Oct 2012 | 5-1 v Bishop's StortfordGloucester City 5-1 Bishop's Stortford | Blue Square Bet North |
| 6 | Sat 13 Oct 2012 | 1-2 v Bradford Park AvenueBradford Park Avenue 2-1 Gloucester City | Blue Square Bet North |
| 7 | Tue 12 Feb 2013 | 162-2 v GuiseleyGloucester City 2-2 Guiseley | Blue Square Bet North |
| 8 | Sat 2 Mar 2013 | 161-0 v WorkingtonWorkington 0-1 Gloucester City | Blue Square Bet North |
| 9 | Tue 5 Mar 2013 | 160-4 v Boston UnitedBoston United 4-0 Gloucester City | Blue Square Bet North |
| 10 | Sat 9 Mar 2013 | 164-1 v Hinckley UnitedGloucester City 4-1 Hinckley United | Blue Square Bet North |
| 11 | Tue 12 Mar 2013 | 164-0 v DroylsdenGloucester City 4-0 Droylsden | Blue Square Bet North |
| 12 | Sat 30 Mar 2013 | 161-1 v HistonGloucester City 1-1 Histon | Blue Square Bet North |
| 13 | Wed 3 Apr 2013 | 162-1 v Bishop's StortfordBishop's Stortford 1-2 Gloucester City | Blue Square Bet North |
| 14 | Sat 6 Apr 2013 | 161-1 v Vauxhall MotorsGloucester City 1-1 Vauxhall Motors | Blue Square Bet North |
| 15 | Tue 9 Apr 2013 | 160-1 v Harrogate TownHarrogate Town 1-0 Gloucester City | Blue Square Bet North |
| 16 | Sat 13 Apr 2013 | 160-4 v Stalybridge CelticStalybridge Celtic 4-0 Gloucester City | Blue Square Bet North |
| 17 | Tue 16 Apr 2013 | 161-2 v Gainsborough TrinityGloucester City 1-2 Gainsborough Trinity | Blue Square Bet North |
| 18 | Sat 20 Apr 2013 | 160-1 v Corby TownGloucester City 0-1 Corby Town | Blue Square Bet North |
| 19 | Sat 27 Apr 2013 | 160-1 v Colwyn BayColwyn Bay 1-0 Gloucester City | Blue Square Bet North |
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