| 1 | Sat 18 Aug 2012 | 1-0 v Gainsborough TrinityGainsborough Trinity 0-1 Gloucester City | Blue Square Bet North |
| 2 | Tue 21 Aug 2012 | 0-1 v Oxford CityGloucester City 0-1 Oxford City | Blue Square Bet North |
| 3 | Sat 25 Aug 2012 | 1-2 v HistonHiston 2-1 Gloucester City | Blue Square Bet North |
| 4 | Mon 27 Aug 2012 | 1-1 v Solihull MoorsGloucester City 1-1 Solihull Moors | Blue Square Bet North |
| 5 | Sat 1 Sep 2012 | 1-3 v GuiseleyGuiseley 3-1 Gloucester City | Blue Square Bet North |
| 6 | Sat 8 Sep 2012 | 4-3 v Stalybridge CelticGloucester City 4-3 Stalybridge Celtic | Blue Square Bet North |
| 7 | Sat 15 Sep 2012 | 0-5 v FC Halifax TownFC Halifax Town 5-0 Gloucester City | Blue Square Bet North |
| 8 | Sat 29 Sep 2012 | 0-1 v DroylsdenDroylsden 1-0 Gloucester City | Blue Square Bet North |
| 9 | Tue 2 Oct 2012 | 5-1 v Bishop's StortfordGloucester City 5-1 Bishop's Stortford | Blue Square Bet North |
| 10 | Sat 13 Oct 2012 | 1-2 v Bradford Park AvenueBradford Park Avenue 2-1 Gloucester City | Blue Square Bet North |
| 11 | Sat 27 Oct 2012 | 3-0 v Hinckley UnitedHinckley United 0-3 Gloucester City | Blue Square Bet North |
| 12 | Wed 31 Oct 2012 | 2-3 v Corby TownCorby Town 3-2 Gloucester City | Blue Square Bet North |
| 13 | Wed 14 Nov 2012 | 40-2 v Leyton OrientGloucester City 0-2 Leyton Orient | FA Cup with Budweiser First Round |
| 14 | Sun 18 Nov 2012 | 40-1 v ChesterGloucester City 0-1 Chester | Blue Square Bet North |
| 15 | Wed 5 Dec 2012 | 41-0 v Boston UnitedGloucester City 1-0 Boston United | Blue Square Bet North |
| 16 | Sat 8 Dec 2012 | 40-1 v WorkingtonGloucester City 0-1 Workington | Blue Square Bet North |
| 17 | Sat 15 Dec 2012 | 42-1 v Vauxhall MotorsVauxhall Motors 1-2 Gloucester City | Blue Square Bet North |
| 18 | Tue 1 Jan 2013 | 44-2 v Worcester CityGloucester City 4-2 Worcester City | Blue Square Bet North |
| 19 | Sat 2 Feb 2013 | 40-2 v AltrinchamAltrincham 2-0 Gloucester City | Blue Square Bet North |
| 20 | Tue 5 Feb 2013 | 41-0 v Brackley TownBrackley Town 0-1 Gloucester City | Blue Square Bet North |
| 21 | Sat 9 Feb 2013 | 41-0 v Bradford Park AvenueGloucester City 1-0 Bradford Park Avenue | Blue Square Bet North |
| 22 | Tue 12 Feb 2013 | 42-2 v GuiseleyGloucester City 2-2 Guiseley | Blue Square Bet North |
| 23 | Tue 19 Feb 2013 | 41-2 v FC Halifax TownGloucester City 1-2 FC Halifax Town | Blue Square Bet North |
| 24 | Sat 23 Feb 2013 | 40-2 v Harrogate TownGloucester City 0-2 Harrogate Town | Blue Square Bet North |
| 25 | Wed 27 Mar 2013 | 42-2 v Colwyn BayGloucester City 2-2 Colwyn Bay | Blue Square Bet North |
| 26 | Sat 30 Mar 2013 | 41-1 v HistonGloucester City 1-1 Histon | Blue Square Bet North |
| 27 | Mon 1 Apr 2013 | 43-2 v Solihull MoorsSolihull Moors 2-3 Gloucester City | Blue Square Bet North |
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