| 1 | Sat 18 Aug 2012 | 381-0 v Gainsborough TrinityGainsborough Trinity 0-1 Gloucester City | Blue Square Bet North |
| 2 | Tue 21 Aug 2012 | 380-1 v Oxford CityGloucester City 0-1 Oxford City | Blue Square Bet North |
| 3 | Sat 25 Aug 2012 | 381-2 v HistonHiston 2-1 Gloucester City | Blue Square Bet North |
| 4 | Mon 27 Aug 2012 | 1-1 v Solihull MoorsGloucester City 1-1 Solihull Moors | Blue Square Bet North |
| 5 | Sat 1 Sep 2012 | 1-3 v GuiseleyGuiseley 3-1 Gloucester City | Blue Square Bet North |
| 6 | Tue 4 Sep 2012 | 1-4 v Brackley TownGloucester City 1-4 Brackley Town | Blue Square Bet North |
| 7 | Sat 15 Sep 2012 | 0-5 v FC Halifax TownFC Halifax Town 5-0 Gloucester City | Blue Square Bet North |
| 8 | Sat 29 Sep 2012 | 0-1 v DroylsdenDroylsden 1-0 Gloucester City | Blue Square Bet North |
| 9 | Sat 13 Oct 2012 | 1-2 v Bradford Park AvenueBradford Park Avenue 2-1 Gloucester City | Blue Square Bet North |
| 10 | Sat 27 Oct 2012 | 3-0 v Hinckley UnitedHinckley United 0-3 Gloucester City | Blue Square Bet North |
| 11 | Wed 31 Oct 2012 | 2-3 v Corby TownCorby Town 3-2 Gloucester City | Blue Square Bet North |
| 12 | Wed 14 Nov 2012 | 140-2 v Leyton OrientGloucester City 0-2 Leyton Orient | FA Cup with Budweiser First Round |
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