| 1 | Sat 15 Dec 2012 | 2-1 v Vauxhall MotorsVauxhall Motors 1-2 Gloucester City | Blue Square Bet North |
| 2 | Wed 26 Dec 2012 | 1-0 v Worcester CityWorcester City 0-1 Gloucester City | Blue Square Bet North |
| 3 | Tue 1 Jan 2013 | 4-2 v Worcester CityGloucester City 4-2 Worcester City | Blue Square Bet North |
| 4 | Sat 2 Feb 2013 | 0-2 v AltrinchamAltrincham 2-0 Gloucester City | Blue Square Bet North |
| 5 | Tue 5 Feb 2013 | 1-0 v Brackley TownBrackley Town 0-1 Gloucester City | Blue Square Bet North |
| 6 | Sat 9 Feb 2013 | 1-0 v Bradford Park AvenueGloucester City 1-0 Bradford Park Avenue | Blue Square Bet North |
| 7 | Tue 12 Feb 2013 | 2-2 v GuiseleyGloucester City 2-2 Guiseley | Blue Square Bet North |
| 8 | Sat 16 Feb 2013 | 2-1 v Oxford CityOxford City 1-2 Gloucester City | Blue Square Bet North |
| 9 | Tue 19 Feb 2013 | 1-2 v FC Halifax TownGloucester City 1-2 FC Halifax Town | Blue Square Bet North |
| 10 | Sat 23 Feb 2013 | 0-2 v Harrogate TownGloucester City 0-2 Harrogate Town | Blue Square Bet North |
| 11 | Tue 26 Feb 2013 | 0-0 v AltrinchamGloucester City 0-0 Altrincham | Blue Square Bet North |
| 12 | Sat 2 Mar 2013 | 1-0 v WorkingtonWorkington 0-1 Gloucester City | Blue Square Bet North |
| 13 | Tue 5 Mar 2013 | 0-4 v Boston UnitedBoston United 4-0 Gloucester City | Blue Square Bet North |
| 14 | Sat 9 Mar 2013 | 4-1 v Hinckley UnitedGloucester City 4-1 Hinckley United | Blue Square Bet North |
| 15 | Tue 12 Mar 2013 | 4-0 v DroylsdenGloucester City 4-0 Droylsden | Blue Square Bet North |
| 16 | Wed 27 Mar 2013 | 2-2 v Colwyn BayGloucester City 2-2 Colwyn Bay | Blue Square Bet North |
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