| 1 | Sat 29 Sep 2012 | 0-1 v DroylsdenDroylsden 1-0 Gloucester City | Blue Square Bet North |
| 2 | Tue 2 Oct 2012 | 5-1 v Bishop's StortfordGloucester City 5-1 Bishop's Stortford | Blue Square Bet North |
| 3 | Sat 13 Oct 2012 | 1-2 v Bradford Park AvenueBradford Park Avenue 2-1 Gloucester City | Blue Square Bet North |
| 4 | Sat 27 Oct 2012 | 3-0 v Hinckley UnitedHinckley United 0-3 Gloucester City | Blue Square Bet North |
| 5 | Wed 31 Oct 2012 | 2-3 v Corby TownCorby Town 3-2 Gloucester City | Blue Square Bet North |
| 6 | Wed 14 Nov 2012 | 30-2 v Leyton OrientGloucester City 0-2 Leyton Orient | FA Cup with Budweiser First Round |
| 7 | Wed 5 Dec 2012 | 31-0 v Boston UnitedGloucester City 1-0 Boston United | Blue Square Bet North |
| 8 | Sat 8 Dec 2012 | 30-1 v WorkingtonGloucester City 0-1 Workington | Blue Square Bet North |
| 9 | Sat 15 Dec 2012 | 32-1 v Vauxhall MotorsVauxhall Motors 1-2 Gloucester City | Blue Square Bet North |
| 10 | Wed 26 Dec 2012 | 31-0 v Worcester CityWorcester City 0-1 Gloucester City | Blue Square Bet North |
| 11 | Sat 2 Feb 2013 | 30-2 v AltrinchamAltrincham 2-0 Gloucester City | Blue Square Bet North |
| 12 | Tue 5 Feb 2013 | 31-0 v Brackley TownBrackley Town 0-1 Gloucester City | Blue Square Bet North |
| 13 | Sat 9 Feb 2013 | 31-0 v Bradford Park AvenueGloucester City 1-0 Bradford Park Avenue | Blue Square Bet North |
| 14 | Tue 12 Feb 2013 | 32-2 v GuiseleyGloucester City 2-2 Guiseley | Blue Square Bet North |
| 15 | Sat 16 Feb 2013 | 32-1 v Oxford CityOxford City 1-2 Gloucester City | Blue Square Bet North |
| 16 | Tue 19 Feb 2013 | 31-2 v FC Halifax TownGloucester City 1-2 FC Halifax Town | Blue Square Bet North |
| 17 | Sat 23 Feb 2013 | 30-2 v Harrogate TownGloucester City 0-2 Harrogate Town | Blue Square Bet North |
| 18 | Tue 26 Feb 2013 | 30-0 v AltrinchamGloucester City 0-0 Altrincham | Blue Square Bet North |
| 19 | Sat 2 Mar 2013 | 31-0 v WorkingtonWorkington 0-1 Gloucester City | Blue Square Bet North |
| 20 | Tue 5 Mar 2013 | 30-4 v Boston UnitedBoston United 4-0 Gloucester City | Blue Square Bet North |
| 21 | Sat 9 Mar 2013 | 34-1 v Hinckley UnitedGloucester City 4-1 Hinckley United | Blue Square Bet North |
| 22 | Tue 12 Mar 2013 | 34-0 v DroylsdenGloucester City 4-0 Droylsden | Blue Square Bet North |
| 23 | Sat 30 Mar 2013 | 31-1 v HistonGloucester City 1-1 Histon | Blue Square Bet North |
| 24 | Mon 1 Apr 2013 | 33-2 v Solihull MoorsSolihull Moors 2-3 Gloucester City | Blue Square Bet North |
| 25 | Wed 3 Apr 2013 | 32-1 v Bishop's StortfordBishop's Stortford 1-2 Gloucester City | Blue Square Bet North |
| 26 | Sat 13 Apr 2013 | 30-4 v Stalybridge CelticStalybridge Celtic 4-0 Gloucester City | Blue Square Bet North |
| 27 | Tue 16 Apr 2013 | 31-2 v Gainsborough TrinityGloucester City 1-2 Gainsborough Trinity | Blue Square Bet North |
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