| 1 | Sat 17 Aug 2013 | 1-2 v Vauxhall MotorsVauxhall Motors 2-1 Gainsborough Trinity | Skrill North |
| 2 | Tue 20 Aug 2013 | 1-2 v GuiseleyGainsborough Trinity 1-2 Guiseley | Skrill North |
| 3 | Sat 24 Aug 2013 | 0-2 v Stalybridge CelticGainsborough Trinity 0-2 Stalybridge Celtic | Skrill North |
| 4 | Mon 26 Aug 2013 | 0-2 v HistonHiston 2-0 Gainsborough Trinity | Skrill North |
| 5 | Sat 31 Aug 2013 | 1-3 v AFC Telford UnitedGainsborough Trinity 1-3 AFC Telford United | Skrill North |
| 6 | Sat 7 Sep 2013 | 1-3 v Stockport CountyStockport County 3-1 Gainsborough Trinity | Skrill North |
| 7 | Sat 14 Sep 2013 | 2-1 v BarrowGainsborough Trinity 2-1 Barrow | Skrill North |
| 8 | Tue 17 Sep 2013 | 2-2 v Worcester CityWorcester City 2-2 Gainsborough Trinity | Skrill North |
| 9 | Sat 21 Sep 2013 | 1-0 v LeamingtonLeamington 0-1 Gainsborough Trinity | Skrill North |
| 10 | Sat 5 Oct 2013 | 2-0 v Harrogate TownGainsborough Trinity 2-0 Harrogate Town | Skrill North |
| 11 | Sat 19 Oct 2013 | 2-3 v Solihull MoorsSolihull Moors 3-2 Gainsborough Trinity | Skrill North |
| 12 | Sat 2 Nov 2013 | 2-4 v WorkingtonWorkington 4-2 Gainsborough Trinity | Skrill North |
| 13 | Sat 9 Nov 2013 | 4-2 v Bradford Park AvenueGainsborough Trinity 4-2 Bradford Park Avenue | Skrill North |
| 14 | Tue 12 Nov 2013 | 0-1 v Boston UnitedGainsborough Trinity 0-1 Boston United | Skrill North |
| 15 | Sat 23 Nov 2013 | 2-0 v Colwyn BayColwyn Bay 0-2 Gainsborough Trinity | Skrill North |
| 16 | Sat 7 Dec 2013 | 6-0 v BarrowBarrow 0-6 Gainsborough Trinity | Skrill North |
| 17 | Tue 10 Dec 2013 | 2-2 v Brackley TownGainsborough Trinity 2-2 Brackley Town | Skrill North |
| 18 | Sat 14 Dec 2013 | 1-1 v Vauxhall MotorsGainsborough Trinity 1-1 Vauxhall Motors | Skrill North |
| 19 | Sat 21 Dec 2013 | 1-0 v Gloucester CityGloucester City 0-1 Gainsborough Trinity | Skrill North |
| 20 | Wed 1 Jan 2014 | 0-2 v North Ferriby UnitedNorth Ferriby United 2-0 Gainsborough Trinity | Skrill North |
| 21 | Sat 4 Jan 2014 | 1-5 v Stockport CountyGainsborough Trinity 1-5 Stockport County | Skrill North |
| 22 | Tue 7 Jan 2014 | 5-4 v AltrinchamGainsborough Trinity 5-4 Altrincham | Skrill North |
| 23 | Sat 11 Jan 2014 | 1-3 v Brackley TownBrackley Town 3-1 Gainsborough Trinity | Skrill North |
| 24 | Sat 18 Jan 2014 | 3-0 v WorkingtonGainsborough Trinity 3-0 Workington | Skrill North |
| 25 | Sat 25 Jan 2014 | 0-2 v Colwyn BayGainsborough Trinity 0-2 Colwyn Bay | Skrill North |
| 26 | Sat 1 Feb 2014 | 0-6 v Boston UnitedBoston United 6-0 Gainsborough Trinity | Skrill North |
| 27 | Sat 8 Feb 2014 | 1-1 v LeamingtonGainsborough Trinity 1-1 Leamington | Skrill North |
| 28 | Sat 22 Feb 2014 | 2-1 v Worcester CityGainsborough Trinity 2-1 Worcester City | Skrill North |
| 29 | Tue 4 Mar 2014 | 2-3 v Solihull MoorsGainsborough Trinity 2-3 Solihull Moors | Skrill North |
| 30 | Sat 8 Mar 2014 | 3-1 v Hednesford TownHednesford Town 1-3 Gainsborough Trinity | Skrill North |
| 31 | Tue 11 Mar 2014 | 1-4 v North Ferriby UnitedGainsborough Trinity 1-4 North Ferriby United | Skrill North |
| 32 | Sat 15 Mar 2014 | 3-3 v Gloucester CityGainsborough Trinity 3-3 Gloucester City | Skrill North |
| 33 | Wed 19 Mar 2014 | 0-4 v Bradford Park AvenueBradford Park Avenue 4-0 Gainsborough Trinity | Skrill North |
| 34 | Sat 22 Mar 2014 | 0-3 v AltrinchamAltrincham 3-0 Gainsborough Trinity | Skrill North |
| 35 | Sat 29 Mar 2014 | 1-1 v Harrogate TownHarrogate Town 1-1 Gainsborough Trinity | Skrill North |
| 36 | Thu 3 Apr 2014 | 0-1 v Oxford CityOxford City 1-0 Gainsborough Trinity | Skrill North |
| 37 | Sat 5 Apr 2014 | 1-2 v Hednesford TownGainsborough Trinity 1-2 Hednesford Town | Skrill North |
| 38 | Tue 8 Apr 2014 | 1-3 v GuiseleyGuiseley 3-1 Gainsborough Trinity | Skrill North |
| 39 | Sat 12 Apr 2014 | 6-0 v Oxford CityGainsborough Trinity 6-0 Oxford City | Skrill North |
| 40 | Mon 21 Apr 2014 | 3-0 v HistonGainsborough Trinity 3-0 Histon | Skrill North |
| 41 | Sat 26 Apr 2014 | 0-3 v AFC Telford UnitedAFC Telford United 3-0 Gainsborough Trinity | Skrill North |
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