| 1 | Sat 17 Aug 2013 | 1-2 v Vauxhall MotorsVauxhall Motors 2-1 Gainsborough Trinity | Skrill North |
| 2 | Tue 20 Aug 2013 | 1-2 v GuiseleyGainsborough Trinity 1-2 Guiseley | Skrill North |
| 3 | Sat 24 Aug 2013 | 0-2 v Stalybridge CelticGainsborough Trinity 0-2 Stalybridge Celtic | Skrill North |
| 4 | Mon 26 Aug 2013 | 0-2 v HistonHiston 2-0 Gainsborough Trinity | Skrill North |
| 5 | Sat 14 Sep 2013 | 2-1 v BarrowGainsborough Trinity 2-1 Barrow | Skrill North |
| 6 | Tue 17 Sep 2013 | 2-2 v Worcester CityWorcester City 2-2 Gainsborough Trinity | Skrill North |
| 7 | Sat 21 Sep 2013 | 1-0 v LeamingtonLeamington 0-1 Gainsborough Trinity | Skrill North |
| 8 | Sat 5 Oct 2013 | 2-0 v Harrogate TownGainsborough Trinity 2-0 Harrogate Town | Skrill North |
| 9 | Sat 19 Oct 2013 | 2-3 v Solihull MoorsSolihull Moors 3-2 Gainsborough Trinity | Skrill North |
| 10 | Sat 2 Nov 2013 | 2-4 v WorkingtonWorkington 4-2 Gainsborough Trinity | Skrill North |
| 11 | Sat 7 Dec 2013 | 6-0 v BarrowBarrow 0-6 Gainsborough Trinity | Skrill North |
| 12 | Sat 14 Dec 2013 | 1-1 v Vauxhall MotorsGainsborough Trinity 1-1 Vauxhall Motors | Skrill North |
| 13 | Wed 1 Jan 2014 | 0-2 v North Ferriby UnitedNorth Ferriby United 2-0 Gainsborough Trinity | Skrill North |
| 14 | Sat 11 Jan 2014 | 1-3 v Brackley TownBrackley Town 3-1 Gainsborough Trinity | Skrill North |
| 15 | Sat 22 Feb 2014 | 2-1 v Worcester CityGainsborough Trinity 2-1 Worcester City | Skrill North |
| 16 | Tue 11 Mar 2014 | 1-4 v North Ferriby UnitedGainsborough Trinity 1-4 North Ferriby United | Skrill North |
| 17 | Sat 15 Mar 2014 | 3-3 v Gloucester CityGainsborough Trinity 3-3 Gloucester City | Skrill North |
| 18 | Wed 19 Mar 2014 | 0-4 v Bradford Park AvenueBradford Park Avenue 4-0 Gainsborough Trinity | Skrill North |
| 19 | Sat 22 Mar 2014 | 0-3 v AltrinchamAltrincham 3-0 Gainsborough Trinity | Skrill North |
| 20 | Tue 8 Apr 2014 | 1-3 v GuiseleyGuiseley 3-1 Gainsborough Trinity | Skrill North |
| 21 | Sat 12 Apr 2014 | 6-0 v Oxford CityGainsborough Trinity 6-0 Oxford City | Skrill North |
| 22 | Mon 21 Apr 2014 | 3-0 v HistonGainsborough Trinity 3-0 Histon | Skrill North |
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