| 1 | Sat 18 Aug 2012 | 0-1 v Gloucester CityGainsborough Trinity 0-1 Gloucester City | Blue Square Bet North |
| 2 | Tue 21 Aug 2012 | 1-1 v Stalybridge CelticStalybridge Celtic 1-1 Gainsborough Trinity | Blue Square Bet North |
| 3 | Sat 25 Aug 2012 | 0-2 v GuiseleyGuiseley 2-0 Gainsborough Trinity | Blue Square Bet North |
| 4 | Mon 27 Aug 2012 | 1-1 v Harrogate TownGainsborough Trinity 1-1 Harrogate Town | Blue Square Bet North |
| 5 | Sat 1 Sep 2012 | 2-1 v Vauxhall MotorsVauxhall Motors 1-2 Gainsborough Trinity | Blue Square Bet North |
| 6 | Sat 29 Sep 2012 | 2-2 v Corby TownGainsborough Trinity 2-2 Corby Town | Blue Square Bet North |
| 7 | Sat 13 Oct 2012 | 3-0 v DroylsdenGainsborough Trinity 3-0 Droylsden | Blue Square Bet North |
| 8 | Sat 20 Oct 2012 | 2-2 v Brackley TownBrackley Town 2-2 Gainsborough Trinity | Blue Square Bet North |
| 9 | Sat 27 Oct 2012 | 1-1 v WorkingtonGainsborough Trinity 1-1 Workington | Blue Square Bet North |
| 10 | Sat 17 Nov 2012 | 5-1 v Bishop's StortfordBishop's Stortford 1-5 Gainsborough Trinity | Blue Square Bet North |
| 11 | Sat 1 Dec 2012 | 3-1 v Colwyn BayGainsborough Trinity 3-1 Colwyn Bay | Blue Square Bet North |
| 12 | Sat 8 Dec 2012 | 1-0 v AltrinchamAltrincham 0-1 Gainsborough Trinity | Blue Square Bet North |
| 13 | Sat 22 Dec 2012 | 1-2 v Stalybridge CelticGainsborough Trinity 1-2 Stalybridge Celtic | Blue Square Bet North |
| 14 | Wed 26 Dec 2012 | 1-3 v FC Halifax TownFC Halifax Town 3-1 Gainsborough Trinity | Blue Square Bet North |
| 15 | Tue 1 Jan 2013 | 3-0 v FC Halifax TownGainsborough Trinity 3-0 FC Halifax Town | Blue Square Bet North |
| 16 | Sat 5 Jan 2013 | 1-1 v Solihull MoorsGainsborough Trinity 1-1 Solihull Moors | Blue Square Bet North |
| 17 | Sat 9 Feb 2013 | 3-1 v DroylsdenDroylsden 1-3 Gainsborough Trinity | Blue Square Bet North |
| 18 | Tue 19 Feb 2013 | 3-0 v HistonHiston 0-3 Gainsborough Trinity | Blue Square Bet North |
| 19 | Tue 26 Mar 2013 | 0-1 v Brackley TownGainsborough Trinity 0-1 Brackley Town | Blue Square Bet North |
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