| 1 | Sat 18 Aug 2012 | 0-1 v Gloucester CityGainsborough Trinity 0-1 Gloucester City | Blue Square Bet North |
| 2 | Tue 21 Aug 2012 | 1-1 v Stalybridge CelticStalybridge Celtic 1-1 Gainsborough Trinity | Blue Square Bet North |
| 3 | Sat 25 Aug 2012 | 0-2 v GuiseleyGuiseley 2-0 Gainsborough Trinity | Blue Square Bet North |
| 4 | Mon 27 Aug 2012 | 1-1 v Harrogate TownGainsborough Trinity 1-1 Harrogate Town | Blue Square Bet North |
| 5 | Sat 1 Sep 2012 | 2-1 v Vauxhall MotorsVauxhall Motors 1-2 Gainsborough Trinity | Blue Square Bet North |
| 6 | Tue 4 Sep 2012 | 2-4 v AltrinchamGainsborough Trinity 2-4 Altrincham | Blue Square Bet North |
| 7 | Sat 8 Sep 2012 | 1-0 v Worcester CityGainsborough Trinity 1-0 Worcester City | Blue Square Bet North |
| 8 | Sat 15 Sep 2012 | 2-0 v Solihull MoorsSolihull Moors 0-2 Gainsborough Trinity | Blue Square Bet North |
| 9 | Sat 29 Sep 2012 | 2-2 v Corby TownGainsborough Trinity 2-2 Corby Town | Blue Square Bet North |
| 10 | Sat 13 Oct 2012 | 3-0 v DroylsdenGainsborough Trinity 3-0 Droylsden | Blue Square Bet North |
| 11 | Sat 20 Oct 2012 | 2-2 v Brackley TownBrackley Town 2-2 Gainsborough Trinity | Blue Square Bet North |
| 12 | Sat 27 Oct 2012 | 1-1 v WorkingtonGainsborough Trinity 1-1 Workington | Blue Square Bet North |
| 13 | Tue 30 Oct 2012 | 5-0 v Hinckley UnitedGainsborough Trinity 5-0 Hinckley United | Blue Square Bet North |
| 14 | Sat 3 Nov 2012 | 1-2 v Boston UnitedBoston United 2-1 Gainsborough Trinity | Blue Square Bet North |
| 15 | Sat 17 Nov 2012 | 5-1 v Bishop's StortfordBishop's Stortford 1-5 Gainsborough Trinity | Blue Square Bet North |
| 16 | Sat 1 Dec 2012 | 3-1 v Colwyn BayGainsborough Trinity 3-1 Colwyn Bay | Blue Square Bet North |
| 17 | Sat 8 Dec 2012 | 1-0 v AltrinchamAltrincham 0-1 Gainsborough Trinity | Blue Square Bet North |
| 18 | Mon 17 Dec 2012 | 2-0 v Bradford Park AvenueBradford Park Avenue 0-2 Gainsborough Trinity | Blue Square Bet North |
| 19 | Tue 1 Jan 2013 | 3-0 v FC Halifax TownGainsborough Trinity 3-0 FC Halifax Town | Blue Square Bet North |
| 20 | Sat 5 Jan 2013 | 1-1 v Solihull MoorsGainsborough Trinity 1-1 Solihull Moors | Blue Square Bet North |
| 21 | Sat 2 Feb 2013 | 2-2 v Boston UnitedGainsborough Trinity 2-2 Boston United | Blue Square Bet North |
| 22 | Sat 9 Feb 2013 | 3-1 v DroylsdenDroylsden 1-3 Gainsborough Trinity | Blue Square Bet North |
| 23 | Wed 27 Feb 2013 | 1-3 v ChesterChester 3-1 Gainsborough Trinity | Blue Square Bet North |
| 24 | Sat 2 Mar 2013 | 2-0 v Hinckley UnitedHinckley United 0-2 Gainsborough Trinity | Blue Square Bet North |
| 25 | Tue 12 Mar 2013 | 1-1 v Bradford Park AvenueGainsborough Trinity 1-1 Bradford Park Avenue | Blue Square Bet North |
| 26 | Sat 16 Mar 2013 | 2-2 v Bishop's StortfordGainsborough Trinity 2-2 Bishop's Stortford | Blue Square Bet North |
| 27 | Mon 18 Mar 2013 | 3-0 v Worcester CityWorcester City 0-3 Gainsborough Trinity | Blue Square Bet North |
| 28 | Tue 26 Mar 2013 | 0-1 v Brackley TownGainsborough Trinity 0-1 Brackley Town | Blue Square Bet North |
| 29 | Sat 30 Mar 2013 | 1-2 v GuiseleyGainsborough Trinity 1-2 Guiseley | Blue Square Bet North |
| 30 | Mon 1 Apr 2013 | 0-0 v Harrogate TownHarrogate Town 0-0 Gainsborough Trinity | Blue Square Bet North |
| 31 | Thu 4 Apr 2013 | 0-2 v ChesterGainsborough Trinity 0-2 Chester | Blue Square Bet North |
| 32 | Sat 6 Apr 2013 | 1-2 v Oxford CityGainsborough Trinity 1-2 Oxford City | Blue Square Bet North |
| 33 | Tue 9 Apr 2013 | 1-0 v HistonGainsborough Trinity 1-0 Histon | Blue Square Bet North |
| 34 | Sat 13 Apr 2013 | 0-1 v Colwyn BayColwyn Bay 1-0 Gainsborough Trinity | Blue Square Bet North |
| 35 | Tue 16 Apr 2013 | 2-1 v Gloucester CityGloucester City 1-2 Gainsborough Trinity | Blue Square Bet North |
| 36 | Sat 20 Apr 2013 | 2-1 v Vauxhall MotorsGainsborough Trinity 2-1 Vauxhall Motors | Blue Square Bet North |
| 37 | Tue 23 Apr 2013 | 1-1 v Oxford CityOxford City 1-1 Gainsborough Trinity | Blue Square Bet North |
| 38 | Sat 27 Apr 2013 | 0-0 v Corby TownCorby Town 0-0 Gainsborough Trinity | Blue Square Bet North |
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