| Second Qualifying Round |
| 20/9/2025 | FT | AFC Varndenians | 2 | 2 | Lydd Town |
| 20/9/2025 | FT | Abbey Rangers(3) | 3 | 1 | (0)Frimley Green |
| 20/9/2025 | FT | Abingdon United(2) | 2 | 3 | (1)Irchester United |
| 20/9/2025 | FT | Alsager Town(2) | 3 | 6 | (2)Coventry United |
| 20/9/2025 | FT | Ardley United(2) | 3 | 2 | (1)Wellingborough Whitworth |
| 20/9/2025 | FT | Armthorpe Welfare(2) | 2 | 6 | (3)AFC Darwen |
| 20/9/2025 | FT | Ashby Ivanhoe | 6 | 1 | Nuneaton Griff |
| 20/9/2025 | FT | Ashford Town (Middx)(0) | 1 | 3 | (1)Kidlington |
| 20/9/2025 | FT | Ashville(1) | 1 | 2 | (2)Penistone Church |
| 20/9/2025 | FT | Athersley Recreation(0) | 0 | 2 | (1)Brigg Town |
| 20/9/2025 | FT | Aylesbury Vale Dynamos(1) | 1 | 3 | (1)London Samurai Rovers |
| 20/9/2025 | FT | Balham(1) | 3 | 2 | (2)Jarvis Brook |
| 20/9/2025 | FT | Barnoldswick Town | 10 | 1 | Sunderland West End |
| 20/9/2025 | FT | Barton Town(0) | 0 | 0 | (0)Retford FC |
| 20/9/2025 | FT | Bearsted(1) | 2 | 2 | (1)Tooting Bec |
| 20/9/2025 | FT | Bedfont(1) | 4 | 0 | (0)Cranfield United |
| 20/9/2025 | FT | Benfleet(2) | 5 | 2 | (0)West Essex |
| 20/9/2025 | FT | Berks County(1) | 5 | 2 | (2)Sandhurst Town |
| 20/9/2025 | FT | Bewdley Town | 1 | 3 | Atherstone Town |
| 20/9/2025 | FT | Billingham Synthonia(2) | 4 | 2 | (1)Tow Law Town |
| 20/9/2025 | FT | Boro Rangers(1) | 1 | 2 | (0)Nelson |
| 20/9/2025 | FT | Broadfields United(1) | 2 | 2 | (2)Wembley |
| 20/9/2025 | FT | Brook House(1) | 1 | 4 | (2)Winslow United |
| 20/9/2025 | FT | Camberley Town(1) | 1 | 1 | (0)SC Thamesmead |
| 20/9/2025 | FT | Chadderton(0) | 4 | 3 | (2)Wakefield |
| 20/9/2025 | FT | Clifton All Whites | 5 | 0 | Pinchbeck United |
| 20/9/2025 | FT | Clipstone Welfare | 0 | 3 | Belper United |
| 20/9/2025 | FT | Corinthian(0) | 3 | 0 | (0)Reigate Priory |
| 20/9/2025 | FT | Cove(1) | 3 | 2 | (1)Alton |
| 20/9/2025 | FT | Crowle Colts(2) | 3 | 2 | (0)Dearne & District |
| 20/9/2025 | FT | Deeping Rangers(0) | 2 | 3 | (1)Aylestone Park |
| 20/9/2025 | FT | Doncaster City(0) | 1 | 4 | (3)Prestwich Heys |
| 20/9/2025 | FT | Dunkirk | 2 | 3 | Harrowby United |
| 20/9/2025 | FT | Easington Sports(3) | 6 | 1 | (0)Holmer Green |
| 20/9/2025 | FT | Eastbourne United | 2 | 0 | Halls AFC |
| 20/9/2025 | FT | Eccleshill United(1) | 1 | 1 | (0)Park View AFC |
| 20/9/2025 | FT | Everett Rovers(0) | 2 | 2 | (0)Staines & Lammas (Middlesex) |
| 20/9/2025 | FT | Eynesbury Rovers | 1 | 0 | Diss Town |
| 20/9/2025 | FT | FC Hartlepool(1) | 1 | 2 | (0)West Allotment Celtic |
| 20/9/2025 | FT | Fisher(1) | 4 | 1 | (1)Sutton Common Rovers |
| 20/9/2025 | FT | Fulwood Amateurs(4) | 5 | 1 | (0)Kendal Town |
| 20/9/2025 | FT | Goole AFC(2) | 4 | 4 | (2)Horbury Town |
| 20/9/2025 | FT | Grangetown Boys Club(1) | 1 | 4 | (3)Tadcaster Albion |
| 20/9/2025 | FT | Greenways(3) | 3 | 2 | (0)Chipstead |
| 20/9/2025 | FT | Gresley Rovers(1) | 4 | 1 | (0)Uttoxeter Town |
| 20/9/2025 | FT | Guernsey(0) | 2 | 0 | (0)Croydon |
| 20/9/2025 | FT | Hallen(6) | 8 | 1 | (0)Milton United |
| 20/9/2025 | FT | Halstead Town(0) | 2 | 1 | (1)Coggeshall Town |
| 20/9/2025 | FT | Harleston Town(0) | 1 | 2 | (0)Wisbech Town |
| 20/9/2025 | FT | Harlow Town(1) | 2 | 2 | (1)Hullbridge Sports |
| 20/9/2025 | FT | Harrogate Railway Athletic(0) | 1 | 1 | (0)Thackley |
| 20/9/2025 | FT | Hilltop(2) | 2 | 2 | (0)Bugbrooke St Michaels |
| 20/9/2025 | FT | Hoddesdon Town(0) | 0 | 2 | (0)Buckhurst Hill |
| 20/9/2025 | FT | Hollands & Blair(0) | 0 | 5 | (2)SE Dons |
| 20/9/2025 | FT | Holmesdale(0) | 1 | 2 | (1)Rochester United |
| 20/9/2025 | FT | Horsham YMCA(0) | 0 | 1 | (1)Snodland Town |
| 20/9/2025 | FT | Hythe Town(1) | 4 | 3 | (1)Athletic Newham |
| 20/9/2025 | FT | Ilford(2) | 3 | 2 | (1)May & Baker |
| 20/9/2025 | FT | Kennington(3) | 4 | 1 | (1)FC Elmstead |
| 20/9/2025 | FT | Knowle(1) | 1 | 2 | (1)Nuneaton Town |
| 20/9/2025 | FT | Leicester St Andrews(0) | 0 | 2 | (2)Eastwood Community |
| 20/9/2025 | FT | Little Oakley(1) | 2 | 0 | (0)Colney Heath |
| 20/9/2025 | FT | Liversedge(1) | 4 | 0 | (0)Parkgate |
| 20/9/2025 | FT | Lutterworth Town | 3 | 2 | Hucknall Town |
| 20/9/2025 | FT | Lydney Town(2) | 3 | 2 | (1)Eversley & California |
| 20/9/2025 | FT | Mendip Broadwalk(0) | 1 | 1 | (0)Reading City |
| 20/9/2025 | FT | Molesey(1) | 2 | 0 | (0)Chessington & Hook United |
| 20/9/2025 | FT | Moulton(1) | 2 | 0 | (0)British Airways |
| 20/9/2025 | FT | Newark and Sherwood United(1) | 1 | 0 | (0)Louth Town |
| 20/9/2025 | FT | Newcastle Benfield(2) | 3 | 0 | (0)Boldon Community Association |
| 20/9/2025 | FT | Newhaven(0) | 1 | 3 | (2)Faversham Strike Force |
| 20/9/2025 | FT | Newport Pagnell Town | 2 | 1 | Kings Langley |
| 20/9/2025 | FT | Northallerton Town | 2 | 0 | Garstang |
| 20/9/2025 | FT | Northampton Sileby Rangers(0) | 2 | 2 | (1)Crawley Green |
| 20/9/2025 | FT | Nostell Miners Welfare(0) | 0 | 4 | (3)Droylsden |
| 20/9/2025 | FT | Peacehaven & Telscombe(4) | 5 | 0 | (0)Westside |
| 20/9/2025 | FT | Phoenix Sports(2) | 4 | 1 | (1)Sporting Bengal United |
| 20/9/2025 | FT | Pickering Town(0) | 0 | 1 | (1)Yarm & Eaglescliffe |
| 20/9/2025 | FT | Pinxton(0) | 0 | 1 | (0)Kimberley Miners Welfare |
| 20/9/2025 | FT | Punjab United(1) | 3 | 1 | (1)Wick |
| 20/9/2025 | FT | Ramsbottom United(3) | 8 | 0 | (0)Glasshoughton Welfare |
| 20/9/2025 | FT | Retford United(3) | 4 | 1 | (1)Bottesford Town |
| 20/9/2025 | FT | Rossington Main(1) | 4 | 0 | (0)Dronfield Town |
| 20/9/2025 | FT | Rusthall(2) | 4 | 1 | (0)Seaford Town |
| 20/9/2025 | FT | Saffron Walden Town(2) | 3 | 0 | (0)Hackney Wick |
| 20/9/2025 | FT | Sandiacre Town | 6 | 0 | Holbeach United |
| 20/9/2025 | FT | Sheerwater(1) | 2 | 1 | (0)Billingshurst |
| 20/9/2025 | FT | Sheffield(0) | 2 | 2 | (1)Burscough |
| 20/9/2025 | FT | Shildon(0) | 3 | 0 | (0)Longridge Town |
| 20/9/2025 | FT | Shirebrook Town(0) | 1 | 2 | (1)Grantham Town |
| 20/9/2025 | FT | Skegness Town(1) | 1 | 2 | (1)Oadby Town |
| 20/9/2025 | FT | Soul Tower Hamlets(1) | 2 | 0 | (0)Bridon Ropes |
| 20/9/2025 | FT | Southwell City | 3 | 5 | Radford |
| 20/9/2025 | FT | Stapleford Town(0) | 0 | 3 | (1)Coalville Town |
| 20/9/2025 | FT | Staplehurst Monarchs(1) | 2 | 0 | (0)Shoreham |
| 20/9/2025 | FT | Staveley Miners Welfare(1) | 2 | 3 | (2)Maltby Main |
| 20/9/2025 | FT | Steyning Town Community | 3 | 0 | Lewisham Borough |
| 20/9/2025 | FT | Sutton Athletic(0) | 2 | 1 | (1)Corinthian-Casuals |
| 20/9/2025 | FT | Thatcham Town(0) | 2 | 1 | (1)Avonmouth |
| 20/9/2025 | FT | Thornaby(0) | 0 | 1 | (0)Jarrow |
| 20/9/2025 | FT | Virginia Water(1) | 2 | 3 | (3)Kempston Rovers |
| 20/9/2025 | FT | Whickham(0) | 1 | 5 | (2)Redcar Town |
| 20/9/2025 | FT | Whitley Bay(1) | 3 | 0 | (0)Knaresborough Town |
| 20/9/2025 | FT | Wokingham Town(0) | 1 | 1 | (1)Cinderford Town |