| Second Qualifying Round | 
|---|
| 20/9/2025 | FT | AFC Varndenians | 2 | 2 | Lydd Town | 
| 20/9/2025 | FT | Abbey Rangers(3) | 3 | 1 | (0)Frimley Green | 
| 20/9/2025 | FT | Abingdon United(2) | 2 | 3 | (1)Irchester United | 
| 20/9/2025 | FT | Alsager Town(2) | 3 | 6 | (2)Coventry United | 
| 20/9/2025 | FT | Ardley United(2) | 3 | 2 | (1)Wellingborough Whitworth | 
| 20/9/2025 | FT | Armthorpe Welfare(2) | 2 | 6 | (3)AFC Darwen | 
| 20/9/2025 | FT | Ashby Ivanhoe | 6 | 1 | Nuneaton Griff | 
| 20/9/2025 | FT | Ashford Town (Middx)(0) | 1 | 3 | (1)Kidlington | 
| 20/9/2025 | FT | Ashville(1) | 1 | 2 | (2)Penistone Church | 
| 20/9/2025 | FT | Athersley Recreation(0) | 0 | 2 | (1)Brigg Town | 
| 20/9/2025 | FT | Aylesbury Vale Dynamos(1) | 1 | 3 | (1)London Samurai Rovers | 
| 20/9/2025 | FT | Balham(1) | 3 | 2 | (2)Jarvis Brook | 
| 20/9/2025 | FT | Barnoldswick Town | 10 | 1 | Sunderland West End | 
| 20/9/2025 | FT | Barton Town(0) | 0 | 0 | (0)Retford FC | 
| 20/9/2025 | FT | Bearsted(1) | 2 | 2 | (1)Tooting Bec | 
| 20/9/2025 | FT | Bedfont(1) | 4 | 0 | (0)Cranfield United | 
| 20/9/2025 | FT | Benfleet(2) | 5 | 2 | (0)West Essex | 
| 20/9/2025 | FT | Berks County(1) | 5 | 2 | (2)Sandhurst Town | 
| 20/9/2025 | FT | Bewdley Town | 1 | 3 | Atherstone Town | 
| 20/9/2025 | FT | Billingham Synthonia(2) | 4 | 2 | (1)Tow Law Town | 
| 20/9/2025 | FT | Boro Rangers(1) | 1 | 2 | (0)Nelson | 
| 20/9/2025 | FT | Broadfields United(1) | 2 | 2 | (2)Wembley | 
| 20/9/2025 | FT | Brook House(1) | 1 | 4 | (2)Winslow United | 
| 20/9/2025 | FT | Camberley Town(1) | 1 | 1 | (0)SC Thamesmead | 
| 20/9/2025 | FT | Chadderton(0) | 4 | 3 | (2)Wakefield | 
| 20/9/2025 | FT | Clifton All Whites | 5 | 0 | Pinchbeck United | 
| 20/9/2025 | FT | Clipstone Welfare | 0 | 3 | Belper United | 
| 20/9/2025 | FT | Corinthian(0) | 3 | 0 | (0)Reigate Priory | 
| 20/9/2025 | FT | Cove(1) | 3 | 2 | (1)Alton | 
| 20/9/2025 | FT | Crowle Colts(2) | 3 | 2 | (0)Dearne & District | 
| 20/9/2025 | FT | Deeping Rangers(0) | 2 | 3 | (1)Aylestone Park | 
| 20/9/2025 | FT | Doncaster City(0) | 1 | 4 | (3)Prestwich Heys | 
| 20/9/2025 | FT | Dunkirk | 2 | 3 | Harrowby United | 
| 20/9/2025 | FT | Easington Sports(3) | 6 | 1 | (0)Holmer Green | 
| 20/9/2025 | FT | Eastbourne United | 2 | 0 | Halls AFC | 
| 20/9/2025 | FT | Eccleshill United(1) | 1 | 1 | (0)Park View AFC | 
| 20/9/2025 | FT | Everett Rovers(0) | 2 | 2 | (0)Staines & Lammas (Middlesex) | 
| 20/9/2025 | FT | Eynesbury Rovers | 1 | 0 | Diss Town | 
| 20/9/2025 | FT | FC Hartlepool(1) | 1 | 2 | (0)West Allotment Celtic | 
| 20/9/2025 | FT | Fisher(1) | 4 | 1 | (1)Sutton Common Rovers | 
| 20/9/2025 | FT | Fulwood Amateurs(4) | 5 | 1 | (0)Kendal Town | 
| 20/9/2025 | FT | Goole AFC(2) | 4 | 4 | (2)Horbury Town | 
| 20/9/2025 | FT | Grangetown Boys Club(1) | 1 | 4 | (3)Tadcaster Albion | 
| 20/9/2025 | FT | Greenways(3) | 3 | 2 | (0)Chipstead | 
| 20/9/2025 | FT | Gresley Rovers(1) | 4 | 1 | (0)Uttoxeter Town | 
| 20/9/2025 | FT | Guernsey(0) | 2 | 0 | (0)Croydon | 
| 20/9/2025 | FT | Hallen(6) | 8 | 1 | (0)Milton United | 
| 20/9/2025 | FT | Halstead Town(0) | 2 | 1 | (1)Coggeshall Town | 
| 20/9/2025 | FT | Harleston Town(0) | 1 | 2 | (0)Wisbech Town | 
| 20/9/2025 | FT | Harlow Town(1) | 2 | 2 | (1)Hullbridge Sports | 
| 20/9/2025 | FT | Harrogate Railway Athletic(0) | 1 | 1 | (0)Thackley | 
| 20/9/2025 | FT | Hilltop(2) | 2 | 2 | (0)Bugbrooke St Michaels | 
| 20/9/2025 | FT | Hoddesdon Town(0) | 0 | 2 | (0)Buckhurst Hill | 
| 20/9/2025 | FT | Hollands & Blair(0) | 0 | 5 | (2)SE Dons | 
| 20/9/2025 | FT | Holmesdale(0) | 1 | 2 | (1)Rochester United | 
| 20/9/2025 | FT | Horsham YMCA(0) | 0 | 1 | (1)Snodland Town | 
| 20/9/2025 | FT | Hythe Town(1) | 4 | 3 | (1)Athletic Newham | 
| 20/9/2025 | FT | Ilford(2) | 3 | 2 | (1)May & Baker | 
| 20/9/2025 | FT | Kennington(3) | 4 | 1 | (1)FC Elmstead | 
| 20/9/2025 | FT | Knowle(1) | 1 | 2 | (1)Nuneaton Town | 
| 20/9/2025 | FT | Leicester St Andrews(0) | 0 | 2 | (2)Eastwood Community | 
| 20/9/2025 | FT | Little Oakley(1) | 2 | 0 | (0)Colney Heath | 
| 20/9/2025 | FT | Liversedge(1) | 4 | 0 | (0)Parkgate | 
| 20/9/2025 | FT | Lutterworth Town | 3 | 2 | Hucknall Town | 
| 20/9/2025 | FT | Lydney Town(2) | 3 | 2 | (1)Eversley & California | 
| 20/9/2025 | FT | Mendip Broadwalk(0) | 1 | 1 | (0)Reading City | 
| 20/9/2025 | FT | Molesey(1) | 2 | 0 | (0)Chessington & Hook United | 
| 20/9/2025 | FT | Moulton(1) | 2 | 0 | (0)British Airways | 
| 20/9/2025 | FT | Newark and Sherwood United(1) | 1 | 0 | (0)Louth Town | 
| 20/9/2025 | FT | Newcastle Benfield(2) | 3 | 0 | (0)Boldon Community Association | 
| 20/9/2025 | FT | Newhaven(0) | 1 | 3 | (2)Faversham Strike Force | 
| 20/9/2025 | FT | Newport Pagnell Town | 2 | 1 | Kings Langley | 
| 20/9/2025 | FT | Northallerton Town | 2 | 0 | Garstang | 
| 20/9/2025 | FT | Northampton Sileby Rangers(0) | 2 | 2 | (1)Crawley Green | 
| 20/9/2025 | FT | Nostell Miners Welfare(0) | 0 | 4 | (3)Droylsden | 
| 20/9/2025 | FT | Peacehaven & Telscombe(4) | 5 | 0 | (0)Westside | 
| 20/9/2025 | FT | Phoenix Sports(2) | 4 | 1 | (1)Sporting Bengal United | 
| 20/9/2025 | FT | Pickering Town(0) | 0 | 1 | (1)Yarm & Eaglescliffe | 
| 20/9/2025 | FT | Pinxton(0) | 0 | 1 | (0)Kimberley Miners Welfare | 
| 20/9/2025 | FT | Punjab United(1) | 3 | 1 | (1)Wick | 
| 20/9/2025 | FT | Ramsbottom United(3) | 8 | 0 | (0)Glasshoughton Welfare | 
| 20/9/2025 | FT | Retford United(3) | 4 | 1 | (1)Bottesford Town | 
| 20/9/2025 | FT | Rossington Main(1) | 4 | 0 | (0)Dronfield Town | 
| 20/9/2025 | FT | Rusthall(2) | 4 | 1 | (0)Seaford Town | 
| 20/9/2025 | FT | Saffron Walden Town(2) | 3 | 0 | (0)Hackney Wick | 
| 20/9/2025 | FT | Sandiacre Town | 6 | 0 | Holbeach United | 
| 20/9/2025 | FT | Sheerwater(1) | 2 | 1 | (0)Billingshurst | 
| 20/9/2025 | FT | Sheffield(0) | 2 | 2 | (1)Burscough | 
| 20/9/2025 | FT | Shildon(0) | 3 | 0 | (0)Longridge Town | 
| 20/9/2025 | FT | Shirebrook Town(0) | 1 | 2 | (1)Grantham Town | 
| 20/9/2025 | FT | Skegness Town(1) | 1 | 2 | (1)Oadby Town | 
| 20/9/2025 | FT | Soul Tower Hamlets(1) | 2 | 0 | (0)Bridon Ropes | 
| 20/9/2025 | FT | Southwell City | 3 | 5 | Radford | 
| 20/9/2025 | FT | Stapleford Town(0) | 0 | 3 | (1)Coalville Town | 
| 20/9/2025 | FT | Staplehurst Monarchs(1) | 2 | 0 | (0)Shoreham | 
| 20/9/2025 | FT | Staveley Miners Welfare(1) | 2 | 3 | (2)Maltby Main | 
| 20/9/2025 | FT | Steyning Town Community | 3 | 0 | Lewisham Borough | 
| 20/9/2025 | FT | Sutton Athletic(0) | 2 | 1 | (1)Corinthian-Casuals | 
| 20/9/2025 | FT | Thatcham Town(0) | 2 | 1 | (1)Avonmouth | 
| 20/9/2025 | FT | Thornaby(0) | 0 | 1 | (0)Jarrow | 
| 20/9/2025 | FT | Virginia Water(1) | 2 | 3 | (3)Kempston Rovers | 
| 20/9/2025 | FT | Whickham(0) | 1 | 5 | (2)Redcar Town | 
| 20/9/2025 | FT | Whitley Bay(1) | 3 | 0 | (0)Knaresborough Town | 
| 20/9/2025 | FT | Wokingham Town(0) | 1 | 1 | (1)Cinderford Town |