| 1 | Sat 6 Aug 2016 | 110-3 v GatesheadGateshead 3-0 Chester | National League |
| 2 | Tue 9 Aug 2016 | 113-0 v Dagenham & RedbridgeChester 3-0 Dagenham & Redbridge | National League |
| 3 | Sat 13 Aug 2016 | 111-3 v Maidstone UnitedChester 1-3 Maidstone United | National League |
| 4 | Tue 16 Aug 2016 | 112-3 v BarrowBarrow 3-2 Chester | National League |
| 5 | Sat 20 Aug 2016 | 111-1 v Boreham WoodBoreham Wood 1-1 Chester | National League |
| 6 | Sat 27 Aug 2016 | 114-0 v Sutton UnitedChester 4-0 Sutton United | National League |
| 7 | Mon 29 Aug 2016 | 111-3 v WokingWoking 3-1 Chester | National League |
| 8 | Sat 3 Sep 2016 | 111-2 v Forest Green RoversChester 1-2 Forest Green Rovers | National League |
| 9 | Sat 10 Sep 2016 | 110-0 v Aldershot TownAldershot Town 0-0 Chester | National League |
| 10 | Tue 13 Sep 2016 | 112-0 v GuiseleyChester 2-0 Guiseley | National League |
| 11 | Sat 24 Sep 2016 | 110-0 v WrexhamWrexham 0-0 Chester | National League |
| 12 | Sat 8 Oct 2016 | 111-0 v Torquay UnitedChester 1-0 Torquay United | National League |
| 13 | Sat 22 Oct 2016 | 111-1 v York CityYork City 1-1 Chester | National League |
| 14 | Tue 25 Oct 2016 | 110-0 v Macclesfield TownMacclesfield Town 0-0 Chester | National League |
| 15 | Sat 29 Oct 2016 | 112-5 v Lincoln CityChester 2-5 Lincoln City | National League |
| 16 | Sat 19 Nov 2016 | 111-1 v BromleyChester 1-1 Bromley | National League |
| 17 | Tue 22 Nov 2016 | 112-2 v SouthportChester 2-2 Southport | National League |
| 18 | Sat 26 Nov 2016 | 113-0 v EastleighEastleigh 0-3 Chester | National League |
| 19 | Tue 29 Nov 2016 | 113-0 v North Ferriby UnitedChester 3-0 North Ferriby United | National League |
| 20 | Sat 3 Dec 2016 | 111-1 v GuiseleyGuiseley 1-1 Chester | National League |
| 21 | Sat 17 Dec 2016 | 112-0 v Aldershot TownChester 2-0 Aldershot Town | National League |
| 22 | Mon 26 Dec 2016 | 112-3 v Solihull MoorsSolihull Moors 3-2 Chester | National League |
| 23 | Sun 1 Jan 2017 | 110-3 v Solihull MoorsChester 0-3 Solihull Moors | National League |
| 24 | Sat 7 Jan 2017 | 112-1 v Braintree TownBraintree Town 1-2 Chester | National League |
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